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Bioaugmentation Contiued..........

  The use of biological additives continued to spread in spite of resistance by the academic community, which believed in the principle of microbial infallability—that indigenous microbes could adapt to and eventually degrade any organic compound that man could produce, without assistance. New strains of bacteria were developed with significant activity in environments that included the great majority of those organic compounds on the EPA “Restricted” list.
  The production methods, then and now, did not involve gene splicing. They consisted of selecting a bacterial strain known to have some activity in the presence of the compound to be degraded, adapting it to progressively higher concentrations of that material, selecting the most active colonies and subjecting them to a process to make the adaptation more permanent. The resultant microbe was then grown in quantity in fermentation vessels and preserved as a liquid suspension or a solid for later applications.
Microbes produced this way have improved the operation of wastewater treatment plants subjected to toxic or inhibitory loads of phenol, formaldehyde, tannins and lignins, chlorinated hydrocarbons, oil refinery wastes and a variety of other organics to which a natural biomass is slow to adapt. They also have been used to restart plants after upsets caused by toxic waste streams or overloading, when the standard practice of seeding with municipal sludge had failed.
  Such documented successes, however, still failed to convince skeptics, who attributed them to anything but bioaugmentation. “Bugs” were too simple and inexpensive a solution to hazardous wastes.
Ironically, a breakthrough occurred in 1978 as a result of a test by Exxon Corp. Exxon operated a split-stream wastewater treatment plant with two identical systems, each receiving exactly the same influent. One side of the plant was augmented with bacteria selected to degrade hydrocarbons while the other maintained its natural biomass. Prior to the test, both systems operated normally, with equivalent reductions in BUD, TOC and selected organics. During the test, there were dramatic improvements in the system receiving bioaugmentation, in all the categories monitored.
The results, in a plant which could be expected to be fully adapted to refinery wastes, led to increased use of bacterial additives in hydrocarbon wastewater processing. The success of microbial additives in treatment plants led in turn to their use for bioremediation.

Bioremediation
  
There is no single bioremediation process. The selection of hydrocarbon-degrading bacteria. With the growing sophistication of bioremediation techniques has come the recognition that microbes are not “infallible” at all; that there are substances that cannot be degraded rapidly without human intervention; and that a variety of environmental conditions must be met for biodegradation to occur at a reasonable rate of speed. These include oxygen concentration, nutrient availability, pH, temperature and moisture. The contaminant’s water solubility and its affinity for soil or another solid matrix also must be considered.
Finally, and perhaps most important, the kinetics of biodegradation of the target materials must be quantified to provide a basis for design and management of the process to be employed.

Continue Reading.... Controlling The Bioremediation Process.......

Continue Reading.... "Controlling The Bioremediation Process"